Dark Field Differential Dynamic Microscopy enables the accurate characterization of the roto-translational dynamics of bacteria and colloidal clusters
arXiv:1710.05669 · doi:10.1088/1361-648X/aa9bc5
Abstract
Micro- and nanoscale objects with anisotropic shape are key components of a variety of biological systems and inert complex materials, and represent fundamental building blocks of novel self-assembly strategies. The time scale of their thermal motion is set by their translational and rotational diffusion coefficients, whose measurement may become difficult for relatively large particles with small optical contrast. Here we show that Dark Field Differential Dynamic Microscopy is the ideal tool for probing the roto-translational Brownian motion of shape anisotropic particles. We demonstrate our approach by successful application to aqueous dispersions of non-motile bacteria and of colloidal aggregates of spherical particles.
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- Protein sizing with Differential Dynamic Microscopy
- Multiple dynamic regimes in a coarsening foam
- The Hitchhiker's Guide to Differential Dynamic Microscopy
- Ab initio uncertainty quantification in scattering analysis of microscopy