Density filtered Fluorescence Correlation Spectroscopy for highly concentrated solutions

Abstract

Fluorescence Correlation Spectroscopy (FCS) is widely used to detect and quantify diffusion processes at the molecular level. The molecules of which diffusion is studied are marked with fluorescent dyes. It is commonly maintained that this technique only applies to systems where the concentration of fluorescent molecules is low. Even if this is the optimal operational condition, we show that FCS can be used also at high concentrations (up to 50$\mu$M) of fluorescent molecules: the detector blinding due to highly fluorescent solutions of concentrated dyes can be avoided by using neutral optic density (OD) filters, and the initial condition of very bad signal to noise ratio (SNR) can be hampered by suitable statistical averaging, as usual in other contexts of signal analysis.

12 pages; 9 figures

References in corpus (3)

• Comparative study of afterpulsing behavior and models in single photon counting avalanche photo diode detectors
• On the role of electrodynamic interactions in long-distance biomolecular recognition
• Experimental detection of long-distance interactions between biomolecules through their diffusion behavior: Numerical study