On the Formation of a Macroscopically Flat Phospholipid Membrane on a Hydrosol Substrate

Abstract

The dependence of the structure of a phospholipid layer (DSPC and SOPC) adsorbed on a hydrosol substrate on the concentration of NaOH in a solution of 5-nm silica particles has been studied by X-ray reflectrometry with the use of synchrotron radiation. Profiles of the electron density (polarizability) have been reconstructed from the experimental data within a model-independent approach. According to these profiles, the thickness of the lipid film can vary from a monolayer (~ 35 Angstrom) to several bilayers (~ 450 Angstrom). At the volume concentration of NaOH of ~ 0.5 mol/L, the film on the hydrosol surface is a macroscopically flat phospholipid membrane (bilayer) with a thickness of ~ 60 Angstrom and with areas of 45 +/- 2 and 49 +/- 3 Angstrom^2 per DSPC and SOPC molecule, respectively.

12 pages, 4 figures, 1 table

References in corpus (3)

• Compact Layer of Alkali Ions at the Surface of Colloidal Silica
• X-ray study of the electric double layer at the n-hexane/nanocolloidal silica interface
• Condensation of Silica Nanoparticles on a Phospholipid Membrane

Cited by in corpus (5)

• Kinetics of the Phospholipid Multilayer Formation at the Surface of the Silica Sol
• Study of DMPS Monolayers on a Water Substrate with Laboratory X-ray Reflectometer
• Laboratory X-ray Study of the Phospholipid Monolayers at the Water Surface
• Modelless X-Ray Scattering Study of a Silica Hydrosol Surface
• Synchrotron X-Ray Reflectivity Study of the Adsorption Film of Octadecanamide at the Toluene-Water Interface