In vivo deep tissue imaging using wavefront shaping optical coherence tomography

Abstract

Multiple light scattering in tissue limits the penetration of optical coherence tomography (OCT) imaging. Here, we present in-vivo OCT imaging of a live mouse using wavefront shaping to enhance the penetration depth. A digital micro-mirror device (DMD) was used in a spectral-domain OCT system for complex wavefront shaping of an incident beam which resulted in the optimal delivery of light energy into deep tissue. Ex-vivo imaging of chicken breasts and mouse ear tissues showed enhancements in the strength of the image signals and the penetration depth, and in-vivo imaging of the tail of a live mouse provided a multilayered structure inside the tissue, otherwise invisible in conventional OCT imaging. Signal enhancements by a factor of 3-7 were acquired for various experimental conditions and samples.

References in corpus (4)

• Recent advances in wavefront shaping techniques for biomedical applications
• Measuring optical transmission matrices by wavefront shaping
• Optogenetic control of cell signaling pathway through scattering skull using wavefront shaping
• Measuring large optical reflection matrices of turbid media

Cited by in corpus (3)

• Collaborative effects of wavefront shaping and optical clearing agent in optical coherence tomography
• Controlling Light Transmission Through Highly Scattering Media Using Semi-Definite Programming as a Phase Retrieval Computation Method
• PhD Thesis: "Ad-hoc control of scattering for adaptive opaque lenses"