Exploiting speckle correlations to improve the resolution of wide-field fluorescence microscopy

Abstract

Fluorescence microscopy is indispensable in nanoscience and biological sciences. The versatility of labeling target structures with fluorescent dyes permits to visualize structure and function at a subcellular resolution with a wide field of view. Due to the diffraction limit, conventional optical microscopes are limited to resolving structures larger than 200 nm. The resolution can be enhanced by near-field and far-field super-resolution microscopy methods. Near-field methods typically have a limited field of view and far-field methods are limited by the involved conventional optics. Here, we introduce a combined high-resolution and wide-field fluorescence microscopy method that improves the resolution of a conventional optical microscope by exploiting correlations in speckle illumination through a randomly scattering high-index medium: Speckle correlation resolution enhancement (SCORE). As a test, we collect two-dimensional fluorescence images of 100-nm diameter dye-doped nanospheres. We demonstrate a deconvolved resolution of 130 nm with a field of view of 10 x 10 ${\text{μm}}^2$.

References in corpus (8)

• Wide-field, high-resolution Fourier ptychographic microscopy
• Non-invasive real-time imaging through scattering layers and around corners via speckle correlations
• Exploiting disorder for perfect focusing
• Image Transmission Through an Opaque Material
• Scattering Lens Resolves sub-100 nm Structures with Visible Light
• Sparsity-based single-shot sub-wavelength coherent diffractive imaging
• Imaging blood cells through scattering biological tissue using speckle scanning microscopy
• Phase Retrieval with Application to Optical Imaging

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• PhD Thesis: "Ad-hoc control of scattering for adaptive opaque lenses"