Effect of nanoclay laponite and pH on the energy transfer between fluorescent dyes

Abstract

Fluorescence resonance energy transfer (FRET) between two dyes acriflavine (Acf) and rhodamine B (RhB) were investigated in solution and Layer-by-Layer (LbL) self assembled films in presence and absence of clay mineral laponite. UV-Vis absorption and fluorescence spectroscopy studies suggest both the dyes present mainly as monomer in solution and films. Energy transfer occurred from Acf to RhB in solution and LbL films. The energy transfer efficiency increases in presence of clay laponite and the maximum efficiency were 78.17% and 32.54% in clay dispersion and in LbL films respectively. Presence of laponite particles onto LbL film was confirmed by atomic force microscopy investigations with a surface coverage of more than 75%. Energy transfer efficiency was pH sensitive and the energy transfer efficiency varies from 4.5% to 44.45% in mixed dye solution for a change in pH from 3.0 to 12.0. With proper calibration it is possible to use the present system under investigation to sense pH over a wide range of pH from 3.0 to 12.0.

28 pages; 5 figures

References in corpus (4)

• Fluorescence resonance energy transfer between organic dyes adsorbed onto nano-clay and Langmuir-Blodgett (LB) films
• Development of a DNA sensor using molecular logic gate
• Preparation and characterization of an anionic dye-polycation molecular films by electrostatic Layer-by-Layer adsorption process
• Langmuir-Blodgett Monolayers of Cationic Dyes in the Presence and Absence of Clay Mineral Layers: N,N'-Dioctadecyl Thiacyanine, Octadecyl Rhodamine B and Laponite

Cited by in corpus (4)

• Formation of fluorescent H aggregates of a cyanine dye in LB films and its effect on energy transfer
• Development of hard water sensor using Fluorescence Resonance Energy Transfer
• Development of an Ion-Sensor using Fluorescence Resonance Energy Transfer
• Sensing of DNA conformation based on change in FRET efficiency between laser dyes