Simultaneous optogenetic manipulation and calcium imaging in freely moving C. elegans

Abstract

A fundamental goal of systems neuroscience is to probe the dynamics of neural activity that drive behavior. Here we present an instrument to simultaneously manipulate neural activity via Channelrhodopsin, monitor neural response via GCaMP3, and observe behavior in freely moving C. elegans. We use the instrument to directly observe the relation between sensory stimuli, interneuron activity and locomotion in the mechanosensory circuit.

Cited by in corpus (8)

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• Neural signal propagation atlas of C. elegans
• Fast deep learning correspondence for neuron tracking and identification in C.elegans using synthetic training
• Information flow through a model of the C. elegans klinotaxis circuit
• Correcting motion induced fluorescence artifacts in two-channel neural imaging
• Closed-loop targeted optogenetic stimulation of C. elegans populations
• Inhibitory feedback from the motor circuit gates mechanosensory processing in C. elegans
• Schrodinger dynamics and Berry phase of undulatory locomotion