Reconstructing an Icosahedral Virus from Single-Particle Diffraction Experiments
arXiv:1107.5212 · doi:10.1364/OE.19.017318
Abstract
The first experimental data from single-particle scattering experiments from free electron lasers (FELs) are now becoming available. The first such experiments are being performed on relatively large objects such as viruses, which produce relatively low-resolution, low-noise diffraction patterns in so-called "diffract-and-destroy" experiments. We describe a very simple test on the angular correlations of measured diffraction data to determine if the scattering is from an icosahedral particle. If this is confirmed, the efficient algorithm proposed can then combine diffraction data from multiple shots of particles in random unknown orientations to generate a full 3D image of the icosahedral particle. We demonstrate this with a simulation for the satellite tobacco necrosis virus (STNV), the atomic coordinates of whose asymmetric unit is given in Protein Data Bank entry 2BUK.
18 pages, 10 figures
References in corpus (2)
Cited by in corpus (9)
- Detecting Orientational Order in Model Systems by X-ray Cross Correlation Methods
- Three dimensional structure from intensity correlations
- The role of the coherence in the cross-correlation analysis of diffraction patterns from two-dimensional dense mono-disperse systems
- Solution of the phase problem for coherent scattering from a disordered system of identical particles
- X-ray cross-correlation analysis of disordered systems: potentials and limitations
- Autocorrelation analysis for cryo-EM with sparsity constraints: Improved sample complexity and projection-based algorithms
- Robust inversion of time-resolved data via forward-optimisation in a trajectory basis
- Bayesian electron density determination from sparse and noisy single-molecule X-ray scattering images
- Structure determination from single-molecule X-ray scattering images using stochastic gradient ascent