Direction dependent mechanical unfolding and Green Fluorescent Protein as a force sensor
arXiv:1107.4188 · doi:10.1103/PhysRevE.84.021918
Abstract
An Ising--like model of proteins is used to investigate the mechanical unfolding of the Green Fluorescent Protein along different directions. When the protein is pulled from its ends, we recover the major and minor unfolding pathways observed in experiments. Upon varying the pulling direction, we find the correct order of magnitude and ranking of the unfolding forces. Exploiting the direction dependence of the unfolding force at equilibrium, we propose a force sensor whose luminescence depends on the applied force.
to appear in Phys Rev E
References in corpus (10)
- Force Dependent Hopping Rates of RNA Hairpins can be Estimated from Accurate Measurement of the Folding Landscapes
- An Ising-Like model for protein mechanical unfolding
- Mechanical unfolding and refolding pathways of ubiquitin
- Changing the mechanical unfolding pathway of FnIII10 by tuning the pulling strength
- Reconstructing the free energy landscape of a polyprotein by single-molecule experiments
- Reconstructing the free energy landscape of a mechanically unfolded model protein
- Protein mechanical unfolding: a model with binary variables
- Unfolding times for proteins in a force clamp
- Equilibrium properties and force-driven unfolding pathways of RNA molecules
- Pathways of mechanical unfolding of FnIII_{10}: low force intermediates