Stretching chimeric DNA: a test for the putative S-form

Abstract

Double-stranded DNA `overstretches' at a pulling force of about 65 pN, increasing in length by a factor of 1.7. The nature of the overstretched state is unknown, despite its considerable importance for DNA's biological function and technological application. Overstretching is thought by some to be a force-induced denaturation, and by others to consist of a transition to an elongated, hybridized state called S-DNA. Within a statistical mechanical model we consider the effect upon overstretching of extreme sequence heterogeneity. `Chimeric' sequences possessing halves of markedly different AT composition elongate under fixed external conditions via distinct, spatially segregated transitions. The corresponding force-extension data display two plateaux at forces whose difference varies with pulling rate in a manner that depends qualitatively upon whether the hybridized S-form is accessible. This observation implies a test for S-DNA that could be performed in experiment. Our results suggest that qualitatively different, spatially segregated conformational transitions can occur at a single thermodynamic state within single molecules of DNA.

References in corpus (6)

• Single-molecule experiments in biological physics: methods and applications
• Theory of High-Force DNA Stretching and Overstretching
• Bending and Base-Stacking Interactions in Double-Stranded Semiflexible Polymer
• Sequence sensitivity of breathing dynamics in heteropolymer DNA
• There and (slowly) back again: Entropy-driven hysteresis in a model of DNA overstretching
• Electronic structure of overstretched DNA

Cited by in corpus (3)

• Coarse-grained simulations of DNA overstretching
• Remembering the work of Phillip L. Geissler: A coda to his scientific trajectory
• Microscopic implications of S-DNA