Characterizing and tracking single colloidal particles with video holographic microscopy
arXiv:0712.1738 · doi:10.1364/OE.15.018275
Abstract
We use digital holographic microscopy and Mie scattering theory to simultaneously characterize and track individual colloidal particles. Each holographic snapshot provides enough information to measure a colloidal sphere's radius and refractive index to within 1%, and simultaneously to measure its three-dimensional position with nanometer in-plane precision and 10 nanometer axial resolution.
6 pages, 3 figures