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4 papers · 2 filters
Improved proteomic analysis of nuclear proteins, as exemplified by the comparison of two myeloïd cell lines nuclear proteomes
Cécile Lelong, Mireille Chevallet, Hélène Diemer +3
One of the challenges of the proteomic analysis by 2D-gel is to visualize the low abundance proteins, particularly those localized in organelles. An additional problem with nuclear…
About the mechanism of interference of silver staining with peptide mass spectrometry
Sophie Richert, Sylvie Luche, Mireille Chevallet +3
The mechanism by which silver staining of proteins in polyacrylamide gels interferes with mass spectrometry of peptides produced by proteolysis has been investigated. It was demons…
Silver Staining of 2D Electrophoresis Gels
Thierry Rabilloud
Silver staining is used to detect proteins after electrophoretic separation on polyacrylamide gels. It -combines excellent sensitivity (in the low nanogram range) with the use of v…
The Whereabouts of 2D Gels in Quantitative Proteomics
Thierry Rabilloud
Two-dimensional gel electrophoresis has been instrumental in the development of proteomics. Although it is no longer the exclusive scheme used for proteomics, its unique features m…