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- Commissariat à l'Énergie Atomique et aux Énergies AlternativesFR116 papers
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5 papers · 2 filters
Fully denaturing two-dimensional electrophoresis of membrane proteins: a critical update
Thierry Rabilloud, Mireille Chevallet, Sylvie Luche +1
The quality and ease of proteomics analysis depends on the performance of the analytical tools used, and thus of the performances of the protein separation tools used to deconvolut…
Sweet silver: A formaldehyde-free silver staining using aldoses as developing agents, with enhanced compatibility with mass spectrometry
Mireille Chevallet, Sylvie Luche, Hélène Diemer +3
Protein detection methods after electrophoresis have to be sensitive, homogeneous, and not to impair downstream analysis of proteins by MS. Speed, low cost, and user friendliness a…
Mitochondrial proteomics: analysis of a whole mitochondrial extract with two-dimensional electrophoresis
Thierry Rabilloud
Mitochondria are complex organelles, and their proteomics analysis requires a combination of techniques. The emphasis in this chapter is made first on mitochondria preparation from…
How shall we use the proteomics toolbox for biomarker discovery?
Pierre Lescuyer, Denis Hochstrasser, Thierry Rabilloud
Biomarker discovery for clinical purposes is one of the major areas in which proteomics is used. However, despite considerable effort, the successes have been relatively scarce. In…
Oxidative stress response: a proteomic view
Thierry Rabilloud, Mireille Chevallet, Sylvie Luche +1
The oxidative stress response is characterized by various effects on a range of biologic molecules. When examined at the protein level, both expression levels and protein modificat…